Seeing fluorescence at super-resolution

Super-resolution fluorescence imaging with single molecules.

The ability to detect, image and localize single molecules optically with high spatial precision by their fluorescence enables an emergent class of super-resolution microscopy methods which have overcome the longstanding diffraction barrier for far-field light-focusing optics. Achieving spatial resolutions of 20-40nm or better in both fixed and living cells, these methods are currently being es...

A guide to super-resolution fluorescence microscopy

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new ap...

Cluster Analysis of Super Resolution Fluorescence Images

Direct Stochastic Optical Reconstruction Microscopy (dSTORM) is a novel, high resolution technique to produce fluorescence images. It uses conventional organic fluorophores which are fluorescent molecules that emit light when excited in either a spontaneous or induced way. Common examples of fluorophores are labeled antibodies, used as markers to indicate locations of certain proteins. The fluo...

Super-resolution optical microscopy: Seeing the smaller picture.

Review of super-resolution fluorescence microscopy for biology.

Several methodologies have been developed over the past several years for super-resolution fluorescence microscopy including saturated structured-illumination microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM). Whi...